eLife27d
JAK inhibition decreases the autoimmune burden in Down syndrome
Staining was followed by a wash in Cell Staining Buffer. Next, cells were permeabilized in Buffer III (Transcription Factor Phospho Buffer Set, BD Pharmingen) for 20 min at 4 °C followed by washing ...
eLife27d
JAK inhibition decreases the autoimmune burden in Down syndrome
Staining was followed by a wash in Cell Staining Buffer. Next, cells were permeabilized in Buffer III (Transcription Factor Phospho Buffer Set, BD Pharmingen) for 20 min at 4°C followed by washing ...
Frontiers1mon
CD28 shapes T cell receptor signaling by regulating Lck dynamics and ZAP70 activation
Human anti-CD3ε (Clone: HIT3a), human anti-CD3ε (Clone: UCHT1), and human anti-CD28 (Clone: CD28.2) monoclonal antibodies were customized for concentration and purchased from BD Pharmingen and were ...
Frontiers3y
Cell-Mediated Immunity to NAGLU Transgene Following Intracerebral Gene Therapy in Children With Mucopolysaccharidosis Type IIIB Syndrome
Lymphocyte subset enumeration was performed by flowcytometry. 50µl of whole blood were mixed with anti-CD16 (clone 3G8, BD Pharmingen), -CD45-PerCP (clone 2D1, BD Biosciences), -CD19-PE-Cy7 (clone ...