The beads were washed with 2% BSA twice and incubated with phycoerythrin-conjugated CD81 antibody (BD Pharmingen; 555676, 1:50) for 1 hour. Following washing, the beads were resuspended in PBS and ...

Antibodies used for flow cytometry were FITC-conjugated anti-human CD3 (RRID : AB 10893003), PE-conjugated anti-human CD4 (RRID : AB_393790), and APC-conjugated anti-human CD8 (RRID : AB_10642579) (BD ...

Immunohistochemical staining was performed using the BrdU In-situ Detection Kit (BD Pharmingen) according to the manufacturer’s protocol. For immunofluorescence, antibodies against Ki67 (D3B5, 1:400, ...

The stained cells were resuspended in 200 μl of PBS containing 100 ng/ml of DAPI (BD Pharmingen, 564907). Flow cytometry was performed using a BD FACSCelesta for data acquisition and a FlowJo V10.7.2 ...

These safe, stable methods can replace traditional radioisotopic techniques for research or high throughput screening in drug discovery. When combined with the TopCount ® Microplate Scintillation and ...

The growth in the number of economic units in Bangladesh has slowed over the past decade, primarily due to capital shortages among rural entrepreneurs, according to the latest Economic Census of ...

For cell sorting, pooled peripheral blood cells from either hTNFtg or WT mice were stained with CD11b, CD115 and Ly6-C antibodies and then isolated using a BD FACSAria II sorter. For in vitro OC ...