BACKGROUND: Thermophilic enzymes are often less active than their mesophilic homologues at low temperatures. One hypothesis to explain this observation is that the extra stabilizing interactions ...

Understanding how enzymes from thermophilic organisms evolved to function at lower temperatures could not only provide insights into evolutionary biology but also aid in bioengineering enzymes ...

In nature, enzymes from microbes that thrive in extremely hot habitats like hydrothermal vents can remain stable even at 100 C. These thermophilic enzymes are useful for the biotech industry ...

A fundamental question is how enzymes can accelerate chemical reactions. Catalysis is not only defined by actual chemical steps, but also by enzyme structure and dynamics. To investigate the role ...

Understanding how enzymes from thermophilic organisms evolved to function at lower temperatures could not only provide insights into evolutionary biology but also aid in bioengineering enzymes ...

HheCas13a from Herbinix hemicellulosilytica was identified as a potential thermophilic enzyme. Its protein sequence was queried for a basic local alignment search tool (BLAST) search against non ...

However, these enzymes are rapidly destroyed when exposed ... the team started to search for [FeFe]-hydrogenases from thermophilic bacteria. Employing bioinformatics tools, they found the ...

This solution utilizes an engineered whole-cell biocatalyst based on the thermophilic bacterium Clostridium ... on developing ...