Staining was followed by a wash in Cell Staining Buffer. Next, cells were permeabilized in Buffer III (Transcription Factor Phospho Buffer Set, BD Pharmingen) for 20 min at 4 °C followed by washing ...
Neuroinflammation, autoinflammation, splenomegaly and anemia caused by bi-allelic mutations in IRAK4
Cells were then incubated with fluorescein isothiocyanate–conjugated (FITC) mouse anti-human CD62L monoclonal antibody 10 μL and phycoerythrin (PE) mouse anti-human CD11b monoclonal antibody 10 μL (BD ...
Lymphocyte subset enumeration was performed by flowcytometry. 50µl of whole blood were mixed with anti-CD16 (clone 3G8, BD Pharmingen), -CD45-PerCP (clone 2D1, BD Biosciences), -CD19-PE-Cy7 (clone ...
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