The beads were washed with 2% BSA twice and incubated with phycoerythrin-conjugated CD81 antibody (BD Pharmingen; 555676, 1:50) for 1 hour. Following washing, the beads were resuspended in PBS and ...

Staining was followed by a wash in Cell Staining Buffer. Next, cells were permeabilized in Buffer III (Transcription Factor Phospho Buffer Set, BD Pharmingen) for 20 min at 4 °C followed by washing ...

Antibodies used for flow cytometry were FITC-conjugated anti-human CD3 (RRID : AB 10893003), PE-conjugated anti-human CD4 (RRID : AB_393790), and APC-conjugated anti-human CD8 (RRID : AB_10642579) (BD ...

Immunohistochemical staining was performed using the BrdU In-situ Detection Kit (BD Pharmingen) according to the manufacturer’s protocol. For immunofluorescence, antibodies against Ki67 (D3B5, 1:400, ...

The stained cells were resuspended in 200 μl of PBS containing 100 ng/ml of DAPI (BD Pharmingen, 564907). Flow cytometry was performed using a BD FACSCelesta for data acquisition and a FlowJo V10.7.2 ...

The government yesterday asked the banks that financed the embattled Beximco Group to submit information regarding the collateral kept by the group against the loans..The directive was given at a ...

For cell sorting, pooled peripheral blood cells from either hTNFtg or WT mice were stained with CD11b, CD115 and Ly6-C antibodies and then isolated using a BD FACSAria II sorter. For in vitro OC ...