The fixed cells were then washed with staining buffer, permeabilized with 0.1% saponin, and stained with antibody for 30 min at 4 °C. Cells were analyzed by LSRII, FACSymphony A3 or FACSCalibur (BD ...
The fixed cells were then washed with staining buffer, permeabilized with 0.1% saponin, and stained with antibody for 30 min at 4 °C. Cells were analyzed by LSRII, FACSymphony A3 or FACSCalibur (BD ...
No writing assistance was utilized in the production of this manuscript. Monoclonal antibodies are novel therapeutic agents used with great success in the treatment of rheumatoid arthritis (RA).
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