Due to the use of dideoxynucleotides in the reactions, Sanger sequencing is also called "dideoxy" sequencing.

Nowadays, Sanger sequencing is usually automated. Automated sequencing instruments combine sequencing with fluorescently labeled primers or dideoxy chain terminators with polyacrylamide gel ...

when read length rarely exceeded 25 bp and attained 80 bp only with the arrival of Fred Sanger's dideoxy terminator chemistry. The sequencing-by-synthesis technology, which uses pyrosequencing for ...

For the HuRef project, the team at JCVI used a more traditional method of sequencing—whole genome shotgun assembly which is built upon Sanger dideoxy sequencing. Then, Applied Biosystems 3730xl ...

Dye-terminator sequencing is performed using a modified protocol supplied by Applied Biosystems BigDye Terminator 1.1 and 3.1 Cycle sequencing kits. All reactions are analyzed using a 48-capillary ...

This guideline specifies recommendations for clinical laboratories that employ amplification and Sanger-based (dideoxy-termination) sequencing of broad-range DNA targets for the identification of ...

Burlingame, June 19, 2024 (GLOBE NEWSWIRE) -- The global sanger sequencing market size was estimated at USD 3,149.9 million in 2024 and is expected to reach around USD 10,130.6 million by 2031 ...

Sanger sequencing is relatively expensive, running about $500 per 1000 bases, compared to less than $0.50 per 1000 bases for next generation sequencing, according to a 2011 study.