Hindgut content was collected by dissecting the crawfish and flushing the intestine with distilled water over a pre-weighed aluminum pan (Figure 3). Abdominal muscle (tail meat) was then extracted.

He placed crawfish in tanks kept at temperatures as low as 50 degrees Fahrenheit and as high as 90 degrees Fahrenheit and added a consistent concentration of ionic lithium to the water. After five ...

After five days, he found that lithium uptake by the abdominal muscle and a part of the crawfish that Doubert did not study -- the animals' exoskeleton -- increased in the warmest tank.